The main components of a UV/Vis spectrophotometer are a light source, a sample holder, a dispersive device to separate the different wavelengths of the light (e. a monochromator), and a suitable detector. A UV-Vis spectrophotometer measures the amount of light that enters. UV-Vis Spectroscopy or Ultraviolet-visible spectroscopy or Ultraviolet-visible spectrophotometer (UV-Vis) is also called absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. Electron transition takes place, so it is also called electron spectroscopy. Its speed, simplicity, and broad applicability make it a core method in research, quality control, and. Ultraviolet spectrophotometry is a powerful technique often employed in various fields of science. In simple terms, the greater the number of absorbing molecules present, the higher the.
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